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The fire affected the basement of the five storey property and crews wearing breathing apparatus rescued three adults from the building - one from the first floor and two from the second floor. All three were taken to hospital by London Ambulance Service suffering from smoke inhalation. A further 200 people left the building before the Brigade arrived.
Station Manager Charles Hanks who was at the scene said: "Our crews worked hard to confine the fire to the basement and to stop it from spreading to the upper floors of the hotel. Firefighters rescued three people and carried out a systematic search of the building to make sure no-one else was inside."
The Brigade was called at 2306 on Friday and the fire was under control by 0156 on Saturday morning.
Crews from Paddington, Kensington, North Kensington, Soho, Hammersmith and West Hampstead fire stations were at the scene. An aerial ladder platform was also in use at the incident.
The cause of the fire is under investigation.